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Metabolic Regulation: Restoration of SCFA production, particularly butyrate, activates G-protein-coupled receptors (GPR41/43), inhibits the NF-B signaling pathway, and reduces the release of pro-inflammatory cytokines such as IL-6 and TNF- (Siddiqui and Cresci, 2021
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Media was then aspirated and cells were washed with PBS (pH 7.4)
