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Samples in the non-anticoagulant tube were solidified at room temperature for 1 h, and serum was collected

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Tyrosine oxidation may also be the result of oxidative stress caused by reactive oxygen and nitrogen species or ultraviolet and visible radiation 10

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Western blot analysis Proteins were extracted from the stored lung tissue samples and A549 cell samples using radioimmunoprecipitation assay (RIPA) lysis buffer (Cell Signaling, US)

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Using multi-omics analysis combined with human ovarian pathology and clinical biopsy, they found that glucose metabolism and tricarboxylic acid cycle (TCA) cycles changed in the granulosa cells of aging patients

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