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Introduction of the amplitude modulation (AM) mode (also known as tapping mode) in 1993, where the cantilever is oscillated in the Z-direction at (or near) resonant frequency, and this oscillation amplitude is measured to detect the tip-sample interaction, facilitated the observation of biomolecular processes allowing analysis of molecules weakly attached to a substrate surface (Zhong et al., 1993)

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(United States: McGraw-Hill Education)

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