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doi: 10.1111/j.1365-2125.2011.03968.x 114 LecailleF.LalmanachG.AndraultP

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Sampson TR, Challis C, Jain N, Moiseyenko A, Ladinsky MS, Shastri GG, et al

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This should not been interpreted as complete absence of oxidative stress, however, since the sensitivity of the methods measuring secondary lipid-peroxidation products is limited as compared with those measuring primary lipid peroxides,207,211,212 and the enzyme, glutathione reductase, may fully account for the conversion of GSSG back to GSH at low ROS levels;213 alternatively, ROS production may be too low to account for effective GSH conversion to GSSG.214 On the contrary, longer ischemic periods (45-90 minutes) followed by reperfusion result in increased lipid peroxidation, even assessing secondary lipid-peroxidation products.87,210,215-220 The impact of this maneuver on both bile-flow generation and the expression of transporters also depends on the duration of the ischemia