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Incapsula incident ID: 1813000670585305889-398927742371172601

Otolaryngol Neck Surg

Immunoblotting and immunoprecipitation assays The cultured cells were rinsed once with ice-cold phosphate-buffered saline (PBS) and lysed in lysis buffer containing 20 mM HEPES (pH 7.0), 1% Triton X-100, 150 mM NaCl, 10% glycerol, 1 mM EDTA, 2 mM EGTA, 1 mM DTT, 5 mM Na 3 VO 4 , 5 mM NaF, 1 mM AEBSF, aprotinin (5 g/ml), and leupeptin (5 g/ml)

The effects of some allyl sulfides in garlic essential oil on cells are listed in Table 2

GSNOR activity was determined by measuring the reaction rate of NADH usage at 340 nm in Ultrospec 3100 pro (Amersham Biosciences) spectrophotometer

[DOI] [PubMed] [Google Scholar] 74.Lanphear BP