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We included 16 absolute assays based on four technologies: (i) amplicon bisulfite sequencing (AmpliconBS) uses next-generation sequencing (NGS) of pooled PCR amplicons derived from bisulfite-converted DNA 31,32,33

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This disrupts the balance between bone resorption and formation, leading to increased bone resorption and reduced bone density

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Western blot assays showed that while KU-57788 did not affect DRP1 Ser616 phosphorylation, it markedly and time- and dose-dependently reduced DRP1-Ser637 phosphorylation in a rapid and sustained manner (Fig

The authors attempted also to elucidate the underlying mechanisms highlighting that H19 directly interacted with the Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (4E-BP1), at the TOS motif inhibiting competitively its binding to Raptor