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Immunoblotting Cells were lysed in 150 uL of ice-cold RIPA buffer supplemented with 1X Complete Mini inhibitor mixture (Roche, #11 836 153 001, Switzerland) and mixed on a rotator at 4C

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When IGF1R is suppressed by siRNA, mouse melanoma cells fail to induce ATM kinase activity after irradiation (Macaulay et al., 2001), suggesting that IGF1R plays a critical role in mediating ATM

PX478 is an inhibitor of HIF-1, which can inhibit the expression of HIF-1 under normal and hypoxic conditions

Nonetheless, these pathogens often rely on repair and detoxifying proteins in addition to these secreted effectors and toxins in order to resist mammalian sources of ROS

International Journal of Molecular Sciences , 26 (8), 3522