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In the fertilized mature oocyte, only the maternal mitochondrial DNA is kept with the mitochondria from the sperm being actively excluded by the zygote [58]

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Preparation of tissue homogenate The testes were excised and washed with saline solution

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Studies in Crat knockout mice demonstrated that CrAT deficiency leads to abnormal fuel selection, which results in a perturbation of glucose homeostasis and suggest that deficits in CrAT activity might contribute to diet-induced metabolic inflexibility by exacerbating the Randle glucose-fatty acid cycle 66