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ROS, such as superoxide anions, hydrogen peroxide, and hydroxyl radicals, are formed as by-products of normal cellular metabolism, especially during the mitochondrial electron transport process [2]

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Hydrogen sulfide (H 2 S), similar to MG, has previously considered as a toxic metabolite, disrupting cell signaling processes, especially those involving ROS [26]

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The next day, the cells were washed 3 times with Tris-phosphate buffer and incubated with the respective secondary antibodies (The Jackson Laboratory) for 2 h