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T-cell costimulation To co stimulate human peripheral T-cells, microplates (Nunc, Wiesbaden, Germany) were pre-coated with goat anti-mouse IgG+M antibody followed by blocking with complete RPMI medium (RPMI+10% FBS), coating with anti-CD3 (20 ng/mL), and anti-CD28 (5 g/mL) antibodies or the respective isotype controls

doi: 10.3389/fnut.2025.1581848 Received 23 February 2025 Accepted 22 September 2025 Published 15 October 2025 Volume 12 - 2025 Edited by Roberta Zupo, University of Bari Aldo Moro, Italy Reviewed by Padhmanand Sudhakar, Kumaraguru College of Technology, India Edem E

2 Thymosin Beta-4 Cardiac Repair Bock-Marquette et al., Nature (2004) This foundational paper demonstrated that the G-actin-sequestering peptide T4 promotes myocardial and endothelial cell migration in the embryonic heart and retains this property in postnatal cardiomyocytes

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