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This step equalizes pressure and prevents vacuum formation, which would otherwise make it difficult to draw liquid and would pull contaminants back through the needle on subsequent draws

Nature 571:515520 BonDurant LD, Ameka M, Naber MC, Markan KR, Idiga SO, Acevedo MR, Walsh SA, Ornitz DM, Potthoff MJ (2017) FGF21 regulates metabolism through adipose-dependent and -independent mechanisms

Autophagy is also an eminently membrane-driven process (Orsi et al

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He L, Zhu C, Jia J, Hao X-Y, Yu X-Y, Liu X-Y, et al

Zhang Z, Zhang X, Tan T (2014) Bioresource technology lipid and carotenoid production by Rhodotorula glutinis under irradiation/high-temperature and dark/low-temperature cultivation