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Methyl esters are the derivative of choice in most applications to the analysis of fatty acids, but when we subject them to electron impact ionization in a mass spectrometer, the charged aliphatic chain breaks up into so many fragments that a branch-point or other structural feature cannot be identified easily

Some types of unsaturated fatty acids cannot be synthesized in mammalian cells, and so must be consumed as part of the diet, such as omega-3

Lewis CA, Brault C, Peck B, Bensaad K, Griffiths B, Mitter R, et al

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Recognizing this gap, my accomplished classmate Ben Marshall T24 and I co-founded the Tuck Europe Club during our first year

This resulted in a reduction of the turnover number to 1.40.4, which, in the absence of thermal deactivation (see below), indicated that the [C-RML][ S 1 ] conjugate could use the limited number of water molecules to complete the reaction cycle via nucleophilic addition to the acylated enzyme intermediate