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EGF (Epidermal Growth Factor) and sh-Oligopeptide-1: the keratinocyte signal Epidermal growth factor was identified in 1962 by Stanley Cohen at Vanderbilt, work that earned the Nobel Prize in 1986

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Cells were recovered by trypsinization and treated with hypotonic (0.56% (w/v)) KCl solution for 15 min

To match the peak steady-state concentration, the loading dose can be calculated from equation (13): TARGET CONCENTRATION INTERVENTION: APPLICATION OF PHARMACOKINETICS & PHARMACODYNAMICS TO DOSE INDIVIDUALIZATION The basic principles outlined above can be applied to the interpretation of clinical drug concentration measurements on the basis of three major pharmacokinetic variables: absorption, clearance, and volume of distribution (and the derived variable, half-life)