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Do not use regular sterile water

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Sirtuin activity declines proportionally with NAD+ levels, reducing stress resistance and promoting cellular senescence

10.1016/j.ejphar.2011.05.038 [DOI] [PubMed] [Google Scholar] Ilic, S

109 Moreover, NAD + -consuming enzymes, such as SIRT3, can also manipulate the cellular redox status via regulating the activity of enzymes for ROS generation and antioxidant factors for ROS eradication