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After harvesting the cells by centrifugation at 4,000 g for 30 min, the cells were resuspended in lysis buffer (50 mM sodium phosphate, pH 8.0, 300 mM NaCl, 10 mM imidazole) and lysed using a sonication for 1 h with a 10-s pulse mode (10 s on and 10 s off) on ice

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doi: 10.1016/j.freeradbiomed.2012.02.043

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A subset of the samples was later thawed for analysis of CS activity using a commercial kit (Citrate Synthase Assay Kit, CS0720, Sigma-Aldrich, St Louis, MO, USA)