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In the experiment, Saccharomyces cerevisiae fungus with an expression plasmid containing the cDNA of the human estrogen receptor and a reporter plasmid having the -galactosidase gene were utilized [7]
After washing the cells with ice cold PBS, HK-2 cells were lysed in lysis buffer supplemented with 1 mM phenylmethylsulfonyl fluoride and protease inhibitor mix

Sekowska A, Kung HF, Danchin A (2000) Sulfur metabolism in Escherichia coli and related bacteria: facts and fiction

doi: 10.1002/anie.200502362

ilin et al

Nonetheless, further longitudinal studies of antioxidant treatment in larger cohorts of biomarker selected patients, controlled by target engagement, are required
