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Acknowledgements Financial support for the work was provided by National Institutes of Health grant nos
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In vitro pacSph transfer assay Purified His-tagged proteins (5 M of sfGFP, STARD3, or NPC2) were incubated with 10 M pacSph in PBS for 60 min at 25C at 500 rpm (Comfort thermomixer, Eppendorf)
