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In our study, we assessed the level of apoptosis in granulosa cells and observed that, in the EZTG group, the expression of Bax and Caspase-3 decreased, while the expression of Bcl-2 increased in the granulosa cells of patients with advanced age.These results suggest that the EZTG formula may suppress GCs apoptosis in advanced-age patients, reduce the number of atretic follicles, and improve ovarian reserve function

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Ras leads to the activation of different mitogen-activated protein kinase (MAPK) cascades, including extracellular-signal-regulated kinase (ERK), p38 MAPK , and c-Jun N-terminal protein kinase (JNK) (Graziani et al., 1993)