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For either double staining of CCK and GAD67 or triple staining of CCK, GAD67, and CaMKII, the free-floating tissue sections were incubated in PBS containing 0.3% Triton X-100 and 5% bovine serum albumin (BSA) at room temperature for 2 h, followed by incubation with a mixture of primary antibodies in PBS containing 5% BSA at 4 C for two nights

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