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Description

Regulation of late G1/S phase transition and APC Cdh1 by reactive oxygen species

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Detection of early and late events in BAX conformational activation To assess the effect of BNIP3-Peptide 1-49 and/or B-017 on the first activation event, recombinant hBAX-10xHis-tagged (400 nM) was incubated with staurosporine (2 M), BNIP3-Peptide 1-49 (5 M), recombinant hPUMA (500 nM), or recombinant hBIM (500 nM) and B-017 (30 M) or the negative control peptide, or recombinant hBCL XL (500 nM), or BSA (5 M) and Pierce Protein A/G magnetic beads-conjugated BAX 6A7 antibody for 1 h