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Immunoprecipitations Cos7 cells co-transfected with indicated plasmids were lysed with ice-cold cell lysis buffer (20 mM Tris-HCl, Ph 8, 130 mM NaCl, 1% Triton X-100, 1 mM DTT, 10 mM NaF, 1 mM phenylmethylsulfonyl fluoride, 10 g/ml aprotinin, 10 g/ml leupeptin, 0.1 mM sodium orthovanadate)

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Front Oncol 14:1359069 Azmal M, Paul JK, Prima FS, Talukder OF, Ghosh A (2024) In silico molecular docking and simulation study to identify potential anticancer phytochemicals targeting the RAS signaling pathway

It may still be safe to take oral Glutathione rather than IV injections