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The membranes were blocked with 5% skim milk in TBS/T (20 mM TrisHCl, pH 7.6, 137 mM NaCl and 0.05% Tween 20) and incubated with an anti-pAkt Ser 473 antibody (#9271

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We found that cuprizone treatment led to a significant increase in the overall level of 6E10-immunoreactvity(-IR) level as well as an increase in the density of cells 6E10 + cells in the soma and proximal dendrites and axon-resembling regions