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For NMR measurements, the samples were re-suspended in 200 L NMR buffer (50 mM sodium phosphate buffer in D 2 O at pH 7.2 with 0.1 mM DSS (4,4-dimethyl-4-silapentane-1-sulfonic acid) for referencing)

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The minor G-allele of the DIO3 variant rs945006 was associated with a protective effect against OA development 105