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The DNA was extracted and purified from the sample using the DNeasy PowerSoil Pro Kit (Qiagen Italia, Milan, Italy)

doi: 10.1111/j.1462-2920.2007.01369.x 233 HsiaoWWMetzCSinghDPRothJ

and NOBLE, R.C

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Chronic systemic inflammation, partly triggered and sustained by cytokines, as well as increased oxidative stress contributes to the pathogenesis of this complex metabolic disorder