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nLDs occur in L4 and adult intestinal nuclei, and can be associated with nuclear ruptures In the above experiments we observed candidate nLDs in the subanterior region of some D1 and D2 intestines, where the nLDs were prominent due to the low level of fat in the surrounding cytoplasm (arrows in Fig 1J, n = 7/104 intestines with nLDs)

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Additionally, if evaluating physiological effects of bioactive compounds, spheroids architecture might difficult an equimolar distribution of bioactive/chemical compounds (including nutrients and metabolites) in the global surface and core of the spheroid structure, with subsequent changes in osmolarity [21]

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