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Homogenates were filtered through clean gauze to remove fat particles and centrifuged at 1,000 g for 5 min to pellet nuclei and cell debris

Below are the criteria for limited flexion (the degree to which one can bend the limb or joint) of the forearm (code 5206)

Lange et al., 2024)

Estimated Familial Amyotrophic Lateral Sclerosis Proportion: A Literature Review and MetaAnalysis. Neurology Genetics 9: e200109

Hiraiwa T, Ito M, Imagawa A, Takamatsu J, Kuma K, Miyauchi A et al (2006) Restriction of dietary Iodine does not ameliorate the early effect of anti-thyroid drug therapy for Graves disease in an area of excessive iodine intake

Wash the glutathione Sepharose column with 10 bed volumes PBS at 1.5 ml/min