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Sections were incubated with 5% BSA for 1 h, followed by primary antibody against GPR68 (1:600, Zen-Bio, Cat No.370126) overnight at 4 C and HRP-conjugated secondary antibody for 1 h at room temperature

In brief, tissues were dissected and directly homogenized in 1 ml of TRIzol reagent on ice

10.1038/s41586-021-03392-8 126 LibbyP.PasterkampG.CreaF.JangI

Failure of autophagy in this context promotes the accumulation of oxidative stress, aberrant signaling, mitochondrial damage, and chronic inflammation, collectively establishing a pro-tumorigenic cellular environment