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For example, certain drugs intended for intrathecal (spinal) injection or neonatal use mandate preservative-free sterile water , because introducing benzyl alcohol or other preservatives in those contexts can be harmful

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Animal experiments A total of 50 8-week-old male Sprague-Dawley (SD) rats were used in the present research and were housed under specific pathogen-free conditions at 23 1.0 C, 50 5% humidity, and 12-h darklight cycle, with free access to water and normal diet

doi: 10.1016/j.yhbeh.2006.06.028