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Trypanosoma cruzi trans-sialidase operates through a covalent sialyl-enzyme intermediate: tyrosine is the catalytic nucleophile

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Immunohistochemistry Paraffin sections of synovial tissues were deparaffinized, rehydrated in descending grades of ethanol, preincubated with BSA for 10 min, and then treated in cold storage overnight with primary antibody (CPT1

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