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Nature 327:779, PMID: 3574469 Hitchler MJ, Domann FE (2007) An epigenetic perspective on the free radical theory of development

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To comprehensively elucidate BSP structures, we employed an integrated analytical platform: UVvisible spectroscopy, gas chromatography (GC), high-performance liquid chromatography (HPLC), size-exclusion chromatography (SEC, including high-performance gel permeation chromatography (HPGPC) variants), GCmass spectrometry (GC-MS), FT-IR spectroscopy, multinuclear NMR ( 1 H, 13C, and 2D), atomic force microscopy (AFM), scanning electron microscopy (SEM), and X-ray diffraction (XRD)