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Even without introducing a post-translation glycosylation option, the hCG subunit could be detected in the Pregnyl sample (reference sample) with a 67% sequence coverage

Both processes are biologically complex and highly context-dependent

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In parallel, we also developed and applied a targeted LC-multiple reaction monitoring (MRM) method to compare the relative quantities of the APF peptide present in urine from both IC/BPS patients and asymptomatic controls (Fig