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Furthermore, glutathione controls the immune response by preserving redox equilibrium inside immune cells, allowing them to operate optimally [92]

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The tissues were counterstained with DAPI (4,6-diamidino-2-phenylindole) for 10 min at room temperature to label the nuclei and then mounted on slides with anti-fade medium (ProLong Gold, Thermo Fisher Scientific Inc., Rockford, IL)

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Oxidative Stress At the end of the experiment, oxidative stress in the collected tissue samples (brain, heart, lung, liver, kidney, stomach, small and large intestine, and blood) was assessed by quantifying the thiobarbituric acid-reactive species (TBARS) as malondialdehyde (MDA) [9,10,12,13,14,19,20]

Peptide Structure Analysis and Sequence Engineering Sequence Optimization Strategies One of the hallmarks of Cagrilintide development is its precise amino acid sequence optimization