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16 Chemistry and Biological Properties of Theanine

Cells were suspended in a 25 L mixture of PBS and Matrigel (1:1, v/v) was injected into the pancreatic tails of mice

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As further support, we analyzed SIRT1-treated SnCE1 C256A AcK231 and SnCE1 Y212A AcK231 by analytical SEC confirming this protein eluting as monomer while non-acetylated SnCE1 C256A eluting as tetramer (Fig