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And definitely dont add any glutathione to your binding buffer save that for the elution step
When you exercise, your body's demand for energy increases, leading to greater utilization of fatty acids, and L-Carnitine supports this process

Kim ve , 2009

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Incubate for 5-10 minutes

A greater reliance on fat for energy can result in a sparing of muscle, an enhancement of exercise performance and a reduction in stored body fat