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The reduced form of nickel binds very poorly to his-tagged proteins, so if you see this color change, youll likely want to stop your purification
Cancer Cell 24 (4), 450465
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[DOI] [PubMed] [Google Scholar] Imamura K, Hishikawa N, Sawada M, Nagatsu T, Yoshida M, Hashizume Y (2003) Distribution of major histocompatibility complex class II-positive microglia and cytokine profile of Parkinsons disease brains
