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Extensive research has highlighted the critical role of the gut-heart axis [93]

Available online at: 145 ChanMKWongMBChernykhVIemeliyanovaMAlvinGNishkumaiOet al

Secondary biological in vitro assay RT-PCR Human hair follicle dermal papilla cells (Promocell, Heidelberg, Germany, used at passages 25) were seeded into 60 mm dishes at a density of 3 10 4 cells/well with DMEM high glucose serum medium supplemented with 10% FBS and 1% antibiotic-antimycotic solution

Additionally, using CD68 KP1, CD68 (PG-M1), and CD163 immunohistochemical antibodies for labeling microglial cells in rats euthanized at 48 h, results were consistent with semiquantitative microglial scoring performed on HE stained slides

Research has also found that theobromine has differential contributions to mood and cognitive function compared to caffeine, providing further insight into the distinct yet synergistic nature of these naturally occurring stimulants [34]