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Mesenchymal stem cell exosomes for the treatment of knee osteoarthritis: current clinical status and future potential

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Briefly, Huh7 cells were lysed in buffer (150 mM KCl, 25 mM Tris pH 7.4, 5 mM EDTA, 0.5 mM DTT, 0.5% NP-40, 9 g ml 1 leupeptin, 9 g ml 1 pepstatin, 10 g ml 1 chymostatin, 3 g ml 1 aprotinin, 1 mM PMSF and 100 U per ml RNase inhibitor) and centrifuged (12,000 g , 30 min, 4 C)

[DOI] [PubMed] [Google Scholar] Bar-Or D, Thomas GW, Rael LT, Lau EP, Winkler JV