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Parasite pellets were then lysed by sonication in citrate buffer (50 mM trisodium citrate [pH 5.5], 0.5% CHAPS, 0.1% Triton X-100, 4 mM dithiothreitol) or phosphate buffered saline (PBS) (pH 7.2)

A fasting inducible switch modulates gluconeogenesis via activator/coactivator exchange