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Notably, the selective uptake of cystine (an oxidized dimer of cysteine), rather than methionine, as a precursor for cysteine and subsequently GSH biosynthesis imposes a further metabolic constraint: the reduction of cystine to cysteine requires NADPH, the supply of which becomes critical for GSH biosynthesis (see Supplementary Fig

For measurement, organelle isolation often exaggerates apparent ROS production by disrupting matrix buffering and catalase activity, so in situ readouts with genetically encoded, peroxisome-targeted reporters (e.g., HyPer or roGFP-Orp1 fused to a PTS1/SKL signal) are preferred to capture authentic, compartment-confined dynamics (Fransen and Lismont 2024), Fig

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Diabetes mellitus decreases hippocampal release of neurotransmitters: an in vivo microdialysis study of awake, freely moving rats

Liver PPARalpha is crucial for whole-body fatty acid homeostasis and is protective against NAFLD