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In overweight cats, supplementation has been associated with increased fatty acid oxidation and enhanced metabolic rate
For Caspase-3 staining, the tissues were blocked with 10% normal horse serum for 30 min then incubated with Caspase-3 antibody (1:300, BD Biosciences, Macquarie Park, NSW, Australia) overnight, followed by secondary antibodies (Goat anti-rabbit HRP, 1:200, Vector Laboratories, Burlingame, CA, USA) for 45 min
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Second, if in doubt, pause it during the active stimulation and egg-collection window and only resume if and when your clinic agrees
doi: 10.1007/s40618-017-0693-z 60 SchomburgL
