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The CDOCKER algorithm was used to dock the ligands, allowing for the generation of ten random ligand conformations, while the heating target temperature was set to 700 K and full potential mode was employed
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Tagged proteins were competitively eluted with Pierce 3x DYKDDDDK peptide and analyzed by western blot ( A ), Pierce Renilla Luciferase Glow Assay ( B ), and silver staining ( C )
