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Total RNA was isolated using an easy-spin TM (DNA Free) Total RNA Extraction Kit (Intron Biotechnology, Co., Ltd., Seongnam, Korea) according to the manufacturers instructions

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72,112,113 Some studies have also shown epitope-dependent pathogenic actions of GAD-Abs in histological brain sections and in vivo preparations, 35,106,114 whereas others showed lack of internalization into hippocampal cultured rat neurons 3 (Table 2)