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2.5.4 CBF analysis CBF was analysed using a system consisting of a high-speed acquisition camera (Sony XCD V60, Tokyo, Japan) connected to an Olympus BX51 microscope with a 5 objective (Tokyo, Japan) and a specific software package (Sony ZCL Viewer, Tokyo, Japan), as previously described ( 2.5.5 MCC analysis MCC was monitored using a high-speed acquisition camera (Sony XCD-U100CR, Tokyo, Japan) connected to a microscope with a with a 5 objective (Olympus BX51), as previously described ( 2.5.6 Mucin secretion measurement Mucin production was quantified using an enzyme-linked lectin assay, following a protocol previously published (Rossner et al., 2019)

doi: 10.1006/abio.1996.0311

doi: 10.1007/BF03345187 51 HernndezJJCGarcaJMMGarca DiezLC

Injection Technique : A longer needle (1 to 1.5 inches) injected at a 90-degree angle

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In the context of metabolic disequilibrium, excessive lipid accumulation and mtROS activate the NLRP3 inflammasome in both hepatocytes and Kupffer cells