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Transepidermal water loss (TEWL) TEWL was measured using Tewameter TM300 (Courage+Khazaka Electronic GmbH, Kln, Germany)

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To get a closer look, we use imaging tests like X-rays or MRIs

RNC-NAC-NMT1 in vitro reconstitution for cryo-EM 100 nM RNCs were incubated with 135 nM wildtype NAC and 135 nM GST-NMT1 in Reconstitution Buffer (20 mM HEPES/KOH pH 7.5, 150 mM KOAc, 5 mM MgCl 2 , 1 mM DTT, 0.05% octaethylene glycol monododecyl ether, 100 mM sucrose) for 1 h on ice before directly preparing cryo-EM grids