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Improving freezethaw tolerance through pre-treatment Before cell freezing, a stearoyl-coA desaturase ( SCD ) 1 inhibitor (CAY10566, Cayman Chemical, MI, USA) was added to the culture medium of MSCs (2.5 M) and HUVECs (25 M) for 3 h

In contrast, animals treated with 200 M neomycin for thirty minutes exhibit dim or completely absent fluorescence