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Description
Inhibitor (2 l) was added to a 96 well microplate in triplicate for each NADPH and inhibitor concentration, and 192 l of the master buffer was added to each well
The prepared samples were analyzed using a Shimadzu LC-2010 A HPLC system with a C18 column (250 mm 4.6 mm, ID 5 m)
Mechanistically, 25-OHC increases membrane permeability through lipid bilayer disorganization, triggering cell death (222-224)
Matching timeline expectations to mechanism biology prevents premature abandonment
Serrano-Macia M
The Role of Glutathione Reductase in Influenza Infection Dentico P, et al
