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SIRT1 or SIRT3 was co-immunoprecipitated and eluted with elution buffer (Pierce # 21004), and their activities were measured using the SIRT GLOTM assay kits (Promega # G6450), according to the manufacturers instructions

Stubborn fat that does not respond to diet and exercise is often a metabolic problem, not a willpower problem

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Signs of an allergic reaction: rash, itching/swelling (especially of the face/tongue/throat), severe dizziness, trouble breathing

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