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doi: 10.3389/fnagi.2017.00121

Isolation of WAT stromal vascular cells and adipocyte differentiation To isolate the stromal vascular fraction, minced iWAT (combined depots from 45 mice per genotype) from 46-week-old mice was digested in 1 mg ml 1 collagenase D (Roche) with 1.5% BSA in sterile Hanks Balanced Salt Solution and incubated in a 37 C shaking water bath for 1 h

Obesity (Silver Spring) 2006;14(Suppl 5 ): 201S7S

Functional enrichment analysis of DEGs was performed using the Database for Annotation, Visualization and Integrated Discovery platform, version 6.8(27), incorporating Gene Ontology (GO

FIGURE 5 Effects of UP on serum metabolites Functioning as phenotype regulators, metabolites were profiled via untargeted metabolism in mouse serum to explore alternations in host metabolism between the CK and UPL groups

So far, many IKK inhibitors displayed significant therapeutic effects in pre-clinical animal experiments, and some inhibitors, such as MLN-120B, IMD-2560, and SAR-113945, have finished the phase I or II clinical trial for the safety, tolerability, and pharmacokinetics