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There's a certain appeal to this reasoning

In particular, 2-oxoglutarate represents a key anaplerotic metabolite linking the entry and exit of carbon atoms from the TCA cycle to pathways involved in amino acid metabolism

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For cell death analysis, 10 5 cells were re-suspended in Annexin buffer (10 mM HEPES, 140 mM NaCl, 2.5 mM CaCl 2 , pH = 7.4) and incubated with Annexin-V-FITC (BD Biosciences, Franklin Lakes, NJ) and PI (Sigma-Aldrich) containing 0.1% Triton X-100 (Sigma-Aldrich) for 15 min at room temperature

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