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The expression of SIRT1 and FoxO3a is significantly reduced in a spontaneous hypertensive rats (SHR) model and the NRK-52E cells exposed to Ang II

In the first step, 200 L of ethylchloroformate was added, then the mixture was shaken for 15 min at room temperature

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Procedure Preheat spectrophotometer/microplate reader for 30 minutes, adjust the wavelength to 412 nm, set zero with distilled Preheat Reagent II in 37 (mammal cell) or 25 (other species) water bath for 30 Blank tube determination: take micro glass cuvette, add 20 L of distilled water, 140 L of Reagent II, 40 L of Reagent III in turn, mix well, place for 2 minutes, and measure 412 nm absorbance AB

Control reactions using protein homogenates from Sf 9 expressing YFP, as well as uninfected Sf 9, were included for all substrates

Glia 28:215224 Harraz MM, Marden JJ, Zhou W et al (2008) SOD1 mutations disrupt redox-sensitive Rac regulation of NADPH oxidase in a familial ALS model